This podcast covers several common separation and purification laboratory techniques that you may see on the MCAT. I have broken this subject down into three different categories: small molecules, proteins, and cells. Within the small molecule category, I cover: extraction, crystallization and recrystallization, and chromatography (TLC and HPLC). In the protein category, I cover: SDS-page, precipitation, isoelectric focusing, and chromatography (size-exclusion, affinity, and ion-exchange). Last, in the cell category, I briefly cover: centrifugation, flow cytometry, and microfluidics.
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