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Separation and Purification Techniques

This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells. 

Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).

For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics. 

Visit MedSchoolCoach.com for more help with the MCAT. 

Jump into the conversation:

[00:00] MCAT Tutorig from MedSchoolCoach

[00:34] Welcome to MCAT Basics

[01:07] Topics covered in this episode

[02:24] Techniques for small molecules – extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)

[28:24] Techniques for proteins – SDS-page, precipitation, isoelectric focusing, and chromatography variations

[54:47] Techniques for cells – centrifugation, flow cytometry, and microfluidics. 

Sam Smith

Sam completed his Bachelors of Science in Chemical and Biological Engineering at the University of Colorado Boulder. Following his graduation, he worked at the National Institutes of Health Vaccine Research Center studying HIV. Meanwhile, with a microphone in his garage, Sam founded the MCAT Basics podcast. The podcast has grown to become the top rated MCAT podcast on iTunes. In addition to podcasting, Sam enjoys the outdoors, sports, and his friends and family.

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